Tuesday, October 15, 2019
Capitalist and Social Democracies & Cause Effect Relationship Essay Example for Free
Capitalist and Social Democracies Cause Effect Relationship Essay Quality of Life The quality of life of nation has always been the most important concern of any of the economic as well as the political theories. And there have been a number of parameters that are required to be looked upon so that quality of life of a nation can be explained on a broader scale. Technical financial terms like GDP per person are very much materialistic in characteristic and hence cannot be considered as a sufficient parameter to indicate the current status of the nationââ¬â¢s quality of life. Some of the literatures have made recognizable adjustments in GDP factor by quantifying other important facets that are left behind while considering GDP measure. The other considerable non market activities are social ills, environmental pollution and many others. But this approach has its own difficulties because it requires the assignment of numerical or monetary values to the various factors that constitute of a wider measure of socio-economic wellbeing and hence a number of processes have been developed to figure out a conclusive remark related to the quality of life while depending on a number of factors that make way for the proposed conclusion. The main purpose of all the attempts has been to make out a list of variables that can be considered as a determinant of quality of life. These measures have undergone a number of selection biases and there have been corresponding arbitrariness in those factors which have been chosen to access quality of life. The indicators that represent those factors have been assigned weights so that a single systematic measure could be achieved. The GDP has its own substantive purpose and despite all its shortcomings it has sufficiently been able to depict clear meaning to the prices that have objective weights for the goods and services that make it up. The proposal suggests that the quality of life can be considered as an outcome depending on nine different factors. The factors and the indicators that are used to represent those factors are: 1. Material wellbeing: It depicts the average GDP of a nation i.e., GDP per person or per capita income. Indicator for this factor has been GDP per person, at PPP in $. It quantifies the very material or average prosperity of the nation. This material well-being is also very importance in enabling elderly people especially senior citizens to retain their independence in later life. 2. Health: This factor has its importance while quantifies the physical as well as mental health of the people. Indicators for this factor are life expectancy at birth and the average age of both male and female. 3. Political Stability and security: The overall development and policy of a nation is a multiple of this factor. The country is being provided ratings while depending on one of the nation as some base. 4. Family life: The condition of a family on an average is an indication of family life of the nation. The divorce rates are being given the status of indicator for quantifying this factor. The divorce rate per 1,000 populations is being converted into index of 1 to 5 with increasing number showing negative trend. 5. Community Life: A factor with value 0 or 1. The null value corresponds lower interest of common public in community life while 1 being assigned to those countries where community life often takes place in form public gathering either at church or trade meetings. 6. Climate and geography: This factor is being indicated through latitude values depicting warmer and colder climatic conditions of the nation. 7. Job Security: This factor is self explanatory and is being indicated through unemployment rate expressed in percentage. 8. Political Freedom: A mean to represent the condition of political and civil liberties with values ranging from 1 to 7 where 1 depicts completely free environment while 7 is provided to an excessively closed political condition . 9. Gender Equality: A very crucial social factor for the quantification of male and female social, economic and financial status with indicator being the ratio of average male and female earnings. The above mentioned variables cover a very broad range of areas that can affect the quality of life of a nation. There a number of other variables that can also affect this quality of life like the education levels, the rate of real GDP growth and income inequality. But in real sense this education level is little correlation between education and life satisfaction and this education level later decides income and finally health. But at the same time, this wellbeing has been found to get under negative influence in case of the job being poorly attuned to academic background of the person as well as its needs and aspirations. Hence this sophisticated tests being done over the above mentioned variables has strong correlation with simple measure of life satisfaction with considerations of both individual and behavioral measures. Cause and effect Analysis The Quality of life has been considered as the function with variables falling in almost six different domains. Under Material Wellbeing, the variables have been none other than the GDP and PPP. These factors are the very reason behind higher state of Material wellbeing. The value of GDP is actually aggregate marketed income of a country and at the same time depicts four different components that measure up to depict well being. The four components are effective per capita consumption flows, net societal accumulation of stocks of productive resources, income distribution poverty and inequality and finally economic insecurity. Health is another domain. The variables that fall under this category are life expectancy at birth i.e., infant mortality and average age. It can be indexed as physical quality-of-life index thereby attempting to measure the quality of life or well being of a nation. The numeric value of the factors associated with it is derived from infant mortality, life expectancy and average age with values ranging from 0 to 100. Family and community is a very important factor which relates the internal condition of a family as well its social participation as an indicator of condition at the level of smallest unit level. The indicators for the quantification of this factor are divorce rate, gender equality and social gathering. Divorce rate is measured per 1000 families while the values related to gender equality and social gathering are decided on arbitrary pattern after comparing the data with some standard nation. Figure (Fishbone diagram for cause and effect analysis Quality of life) Political Stability is another extra personal factor which has its own impact. The countryââ¬â¢s political condition is a sharp reflection of what policies does the country takes for boosting social and political stability. The better the political condition the better will be the future direction. Every thing right from security, freedom, development as well as country and its peopleââ¬â¢s future direction in terms of financial gain and income depends on the political condition. The indicators for the political scenario of a nation are stability, security, freedom etc. which are assigned some value according to the some established norms or formula. Environment is another factor which has a deciding importance in countryââ¬â¢s quality of living. The climate and geography are the indicators which are assigned some value play as variable while determining the quality of life. If we look at the list of nations in between Tropic of Cancer and Tropic of Capricorn, the climatic condition over the region is almost the same and at the same time, almost all of the nations in this particular area are either developing or under developed. Hence the geography and climate has its own impact of quality of life. Apart from all the above mentioned factors, some auxiliary factors are also equally valuable. Job security and inflation are some of the few such indicators making an impact on quality of life. Social Democracy, Capitalist Democracy quality of life Nationââ¬â¢s democratic condition is very much responsible in causing any effect on factors that make way for defining the quality of life. The material well which is indicated by the GDP and PPP is considered to perform strongly in a capital democracy if compared with social democracy. Now more money will get translated into better spending in health care and hence indicators like life expectancy and average life should be expected to be of higher order in case of capitalist democracy but the ground situation is very much different. The worldââ¬â¢s biggest capitalist democracy i.e., US has reformed its welfare programs in the year 1996 but the new provisions in the welfare act failed to provide both health and social benefits to under privileged. The health inequality is at its peak. Despite being one of the biggest spenders in health sector, its performance on health care index is much less than other OECD nations but the personal care policy that has been followed in social economy of United Kingdom and Ireland has helped in giving better results like prolonging life expectancy. The two types of democracies have different philosophies on issues like political freedom, justice and social security. The idea for the development of next generation is to make way for equality of political and economic participation and at the same time basic chances of life for social justice expanded from legal and material preconditions for freedom and equal rights to equal dignity. Political freedom has to be expanded from self determination of government to the taking charge of own life. Capitalist democracy has defined equal opportunity as providing uniformity in life but social democracy expands it to create space for developing personal predilections and talents. à The different approaches for the factors in case of social and capital democracies have made way for separate behavior of the same indicators in determining the performance of the nations on quality of life index. Subject for study The purpose of the proposed paper is to figure out relationship between above mentioned indicators and the quality of life in two different environments with one being capital democracy while the other one being social democracy. The democratic condition of a nation defines the very state of its people and their way of living. The points which have to be figured out in the study of the subject is the effect of the two type of democracies on each of the indicators and then how these changes will cause the change in quality of life of the nation and its people. Measurement Each of the indicators has to be given some numeric value. These values are assigned on specific formulas and techniques that will either be in accordance with those used by UN or if necessary new formulas will be developed. The quality of life is the output function with final value being proportional to the values of the indicators. Data Collection Methods The next step is that of data collection. The data which has to be used for analysis is more of secondary in nature with most of them being categorized into three basic types. â⬠¢ Documentary ââ¬â with material in form written text or non written materials. â⬠¢ Multiple Source ââ¬â area based sources which are basically local journals and country exploration. â⬠¢ Survey ââ¬â public surveys while taking each and every class into account. It can be censuses or ad-hoc surveys The above mentioned types of secondary information have to be extracted from books, journal articles, newspapers magazines, conference papers, UN reports, archives, electronic database, internet, etc. The majority of the data will get utilized in figuring out a complete literature review with information gathered would give details of different indicators of quality of life, factors affecting those indicators and how are these going to behave in case of the two democracies that has been considered for discussion. In the present research we will generally lean on secondary data and the surveys will have to be done in two nations. For Capitalist democracy, United States of America has to be explored while for Social democracy, Ireland has been taken as case study. The details of a public survey can be taken from the surveys of United Nation and other reliable sources and the same could be used for the analysis in this case. Analysis The data obtained through the various methods discussed above would be either quantitative or qualitative. Since the research requires interpretation of data that is likely to be enormous is quantity with proper statistical analysis. Quantitative analysis of the data is more likely to be secondary and exploratory in nature requiring proper summarising. The data that has been obtained through survey would mostly be categorical and will require proper scale based ranking. Qualitative analysis of data is expected to be used more if it is being compared with quantitative analysis. It will require quantification up to certain degree but an overall non-quantifying method is more likely to be used through the data analysis and interpretation process and finally comparison of the final output. This would begin with categorisation according to possible unitisation, making out relations and then developing categories to facilitate it, and developing and testing hypotheses to reach conclusion. Schedule The whole process has to be divided into a number of phases. The first phase is the analysis of the research problem according to the theoretical framework. The maximum required time for first phase analysis is one week. The outcome of this analysis will decide the type of data to be arranged or searched for full fledged analysis. The data search is mainly secondary in nature and the sources for the same are in the form of documentary, journals, research paper and surveys. The important thing is source reliability. There has to be at least two mid term analysis so that quality of the collected data that has been collected so far can be checked. The schedule for the whole project is as follows: Object Statement Analysis : 1 week Data Search (First phase) : 3 weeks Data Analysis (Mid term) : 1 week Data Search (Second phase) : 3 weeks Data Analysis (Mid term) : 1 week Data Search (Third phase) : 3 weeks Data Analysis (Final) : 3 weeks Report Documentation : 1 week Total : 16 weeks Budget The main part of the total expenditure is in making this project will get utilized in data collection. Since the data collection is basically secondary in nature and most of them are freely available so the actual cost to be incurred in the same is in tune of $1000-$1500. So the maximum possible budgetary requirement is around $1500. Conclusion The final report project will have the following format: 1. Title 2. Research Question 3. Literature Review 4. Research Methodology 5. Conceptual Framework 6. Primary Research 7. Analysis 8. Findings 9. Conclusions Recommendations 10. References References National Governors Association (2005), A National Health Care Innovations Program: A proposal to increase the cost-effectiveness and quality of the U.S. health care system. U. S. Department of Health and Human Resources (2003), Steps to a Healthier US: A Program and Policy Perspective. The Power of Prevention. European Observatory on Health Care Systems (1999), Health Care Systems in Transition: United Kingdom, WHO Regional Office, Europe. Osberg, L. Sharpel, A., Comparisons of Trends in GDP and Economic Well-being the Impact of Social Capital Huo, J, Nelson, M. Stephens, J. (2006) Decommodification and Activation in Social Democratic Policy Resolving the Paradox Nueva Sociedad (2007) Social Democracy in the 21st Century Economist Intelligence Unit (2005) Quality-of-life index: The Economist Intelligence Unitââ¬â¢s quality-of-life Index [Online] Available from: http://www.economist.com/media/pdf/QUALITY_OF_LIFE.pdf Campbell, A., Phil C., Rodgers, W. (1976). The Quality of American Life. New York: Russell Sage Foundation. Diener, E., Sandvik, E., Seidlitz, L. Diener, M. (1993) ââ¬Å"The Relationship Between Income and Subjective Well Being: Relative or Absolute? Social Indicators Research. Esping-Andersen, G. (1990) The Three Worlds of Welfare Capitalism. Princeton: Princeton University Press. Frey, B. S. and Alois S.( 2002). Happiness and Economics. Princeton, NJ: Princeton Univeristy Press. Elazar, D. (1984). American Federalism: A view from the states. New York: Thomas Crowell.
Monday, October 14, 2019
Characteristics Of A Good Leader
Characteristics Of A Good Leader There are many things we can be determine a leader whether it is good or bad. The most important thing is the characteristics of the leader. Some of the characteristics of a good and efficient leader are through experience, development and leadership training while the others are born to. Those leaders who have in born characteristics of a good and efficient leadership are usually the ones who become a successful leader. This is true because not everyone can lead. Some people are obedient and dormant by nature, and regularly require someone to overlook their schedule or activities and remind them about the extraordinary goal, so that they will not cut out of leadership. The best leaders are those who are naturally born with good qualities of a leader. The characteristics for a good and efficient leader are self leadership, vision, wise, passionate, compassion, charismatic, great communicator, persistent, integrity and disciplined. No matter it is corporate leadership, team leadership or global leadership, the basic characteristics for a good leader remain the same. Additional positive characteristics may be needed, depending on their field of work. The first characteristics is self leadership, this may be the most important characteristics among the rest because it asserts that only when a leader leads him towards a successful achievement whereas he will have the capabilities to motivate his followers to reach their goals or objectives as well. Besides that, good leaders must have a great vision. This kind of leaders, they know what their objective is and which path they want to go. A good leader will know how to motivate their followers to believe in the same vision that they have for their own country, community and their lives. They will have the different way of viewing of what they could be and not what they are. In addition, to become a good leader, usually they will have to make a critical decision at s ome points in their community or organization. To make the community or organization to become successful, the leader must have the knowledge to make the right decision in order to make the organization to be success. Normally, good leaders must be wise, strategic and perceptive. Furthermore, to become a successful leader, usually, the person is very passionate. They will strongly obsess in whatever they are doing. It could be a hobby, sports or business. They will operate with such a very high level of excitement until they will get addicted in it. Other than that, a good leader will have the compassion for their followers or employees. They have great development and coaching skills. While these leaders have the objectives to accomplish, they will regularly care for the individual that supports them. They are not selfish people but only thinking of their own wants and needs. Most of them have a heart for the individuals that follow them. Besides that, most of the good leaders are usually fascinating. They are very friendly as well because they need to tend to draw other people in with their own personalities. They are distinguished building relationships and they have a great patience of performance from their peers. Moreover, basically, a good leader is usually a great communicator and persuaders. They are very comfortable with communicating with other people and are very inspiring people as well. It is not surprising if they can build a good following with this kind of communication ability. Other than communication, good leaders will also need to determine in achieving their objectives. They know that reaching their destination can be filled with a lot of problems or barriers. So, they find that the advantages of achieving the mission are larger than the problems that they are facing with. This situation makes them strongly persistent person. Besides, they mean what they say. They have integrity. They are the peoples who keep their promises or guarantees and they will not play the old political games that plenty of others people do. People find them trustable and as such are dedicated to them. They also need to be bold sometimes coz they have to be brave to chase after their goals or dream. Al though the fears are real, a daring leader continue to chase after it no matter what regardless fears that exits. Last but not least, to become a good and efficient leader you must have discipline so that you can continue your journey to success. But most of other individuals will simply dispirit or distracted if you will not have the level of discipline. A good leader will discipline their mind to be calm and focused on whatever situation they are facing. In conclusion, not all good leaders can follow the entire step. Also, you may be strong at some points and weak at others. But remember, this world no one will be perfect but the person who finds out or understand his weakness and will make an effort to build up these characteristics of good leadership, this person will achieve or succeed for whatever their objectives or mission is. Question 2 (Describe the qualities you will feel justified in calling an individual a successful person) Success has a very extensive definition. You can be triumphant in terms of career, business, power, health, relationship, and discoveries among the others. We know that life will is full with bumpy and rocky highways and confusing side roads. However, it doesnt mean that you should give up easily and lay the rest all your dreams. Despite the uncertainties, here are the ways that guide you to become a successful person. First of all, a successful person, they know what their objective or mission is. They will not stop until they get what they are aiming for. They keep moving forward and will not turn back. They are more educated and know whats right and whats wrong. They must also have their own vision because vision has the ability to be imaginatively and see our life plans in a clear way and comprehensively understood in our mind. One of the greatest masters of vision is Muhammad Ali because his vision of victory will help him to improve his skills and he will analyze every match that he fights. Besides that, to be a successful person, you must have a very high of focus level. If you ever watch a professional sports player during their matches then you will know what focused people looks like. For example, the world class player Roger Federer, he will only focus on the tennis ball when the opponent strikes back to him. He will not think about others stuff such as, where he will go for his next vac ation, what is he going to eat for dinner or what his children are doing now, he will only focus on the tennis ball and which direction to hit. Other than that, a successful person normally is well educated and will keep on learning new skills to improve their knowledge. Education is also one of the main elements for peoples life and it is the root of success. Therefore, give a full attention or priority to education as there is no substitute of it. Furthermore, a successful person will know how to differentiate what is correct and what is now. We have thousands of decision to make every day. We sometimes will run out of the track because of the number of things that we are thinking about every day. Generally, when we want to start to work on something, and then your friend will call you to go out for lunch n others. You know you need to finish your job or work before you do other things but you will find yourself saying, well, I can take a break awhile and go out for lunch with my friends and end up spending more time at lunch with your friends than you wanted. So now you need to stay back at the office for over time and miss your childs game at home. When you know how to differentiate what is important and what is not, you will find yourself achieving your goals or mission a lot faster, and find yourself doing things that you want to do rather than things you have to do. Besides that, the other method to become a successful pe rson is to associate with people whore successful and positive. In the matter of fact, birds of the same feather flock together. You could `learn a lot with these successful people by communicating and associate with them. They will become your basis of what a successful person is. Other than mixing around with successful people, to be successful in life, you also need to be brave to take the risk to solve whatever problems youre facing in the future. You must always remember the word Never Give Up and dont be afraid to take any challenges. When comes to solving problems, be confident and never surrender in finding the solution to solve the problems that youre facing in otherwise you will never succeed in the future. Moreover, a successful person must have the ability to chase after your dreams in the face of difficulty when others will give up. Example, the man who had an extraordinary persistence was Thomas Edison. He conducted over hundreds of experiments to get electricity to produce light. He kept going with his experiments when others told him to stop saying that with every failure he was one step closer to successes. In addition, to be a successful person in life, you need to be committed in whatever youre doing. When a goal or mission needs to take at least more than six month to achieve it, people who are committed will stay on the track until to the very end. Most people want to achieve their goals or mission in a week or in the other word fast, yes, they can but those are short term goals but Im talking about long term goals or mission. When you come to a long term goal, for sure you will get a little staggering when things is not going the right way as you predict it would be. However , taking the time to see the bigger picture will help you to be motivated and will keep you focused. In conclusion, if you want to be a successful people, you need to be hard working and never stop achieving what you aim for whereas being a successful person means looking inside yourself and ask yourself that you are somebody and you have something to offer. When you are trying to decide whether you are a successful person or not, the first person who must believe that you are a successful person is yourself.
Sunday, October 13, 2019
Confucianism Essay -- Papers China Chinese Culture Religion Essays
Confucianism A philosopher named Confucius founded Confucianism in China 2,500 years ago. Confucianism is a system of ethical behavior and social responsibility that became the great traditions of the East.1 It played an important role in the evolution in Chinese culture over the centuries. It has influenced near-by countries and had made a mark in the history of religion. There are today over six million people who call themselves Confucianists. Most Confucianists live in East Asia where Confucianism is thought to be born. For over two thousand years, Confucianism has been the dominant philosophical system of Chinese government, which has still a great hold on their people. Because Chinese culture has spread to the counties of Vietnam, Korea, and Japan, the values of Confucianism can be found there too. Confucius Confucius was born in 551 BC in the village of Zou in the country of Lu. During that time, the Zhou dynasty was in decline-this was controlled by King Zhou. As a child, Confucius would have make-believe rituals; as a young adult he quickly earned a reputation for politeness, fairness and had a love for learning. He traveled often and studied at the imperial capital, Zhou, where legend has it he spoke with Lao Zi, the leader of Daoism. When Confucius returned to Lu, he became a renowned teacher. At the age of 35, Confucius followed Duke Zhao into war. Duke Zhao was later captured and Confucius again followed. Zhao often went to Confucius for advice, but was later advised not to by one of his advisors. Eventually Zhao stopped going to him for advice and Confucius returned to Lu. When he returned, he saw that conditions have been unchanged so he retired from public life to concentrate on teaching and studying... ...ducted at the many Confucian temples on the island. In June, Taiwan?s Dragon Boat Festival commemorates the death, in 299 BCE, of the Confucian diplomat Chu Yuan, who drowned himself to call attention to unheeded demands for government reform. Bibliography: 1Confucianism ? World Religions by Thomas and Dorothy Hoober P. 3 2 Confucianism ? World Religions by Thomas and Dorothy Hoober P. 75 3 Confucianism http://albany.net/~geenius/kongfuzi/teach.html 4 Confucianism ? Encarta Encyclopedia http://www.encarta.com 5 Confucianism ? World Religions P. 56 6 Confucianism http://www.albany.net/~geenius/kongfuzi/modkong.html 7 World Book Encyclopedia ? Confucianism 8 Confucianism http://www.encyclopedia.com 9 Confucianism http://connect.net/ron/confucianism.html 10 Confucianism http://www.encarta.com (I would check it first)
Saturday, October 12, 2019
Wine Industry Essay -- essays research papers fc
Americaââ¬â¢s winemakers are making superior wines and reaping global acclaim. In a single generation the United States wine industryââ¬â¢s global success is a fascinating story of entrepreneurial vision and savvy marketing. The American industry has new innovations, new competition, and new markets, which make the future look bright for the wine industry. à à à à à In terms of worldwide recognition and success, individual American wineries have made their mark only in the last 50 years. Not until the end of Prohibition in 1933 did the American wine industry take off on a large scale. America has now become the fourth largest producer in the world behind Italy, France, and Spain. The wine market consists of still wine, sparkling wine, and vermouth. Since the 1970ââ¬â¢s, per capita consumption in the United States has grown from 1.3 to 2.7 gallons in 2003. Retail wine sales in the U.S. were a record 21.1 billion. In 2002, Americans consumed 595 million gallons of wine. à à à à à Core wine consumers, about 19.2 million, account for 86% of table wine volume consumed in the U.S. Marginal wine consumers, about 28.9 million, account for 14% of table wine volume consumed in the U.S. Core drinkers are somewhat older than marginal drinkers. 51% are between 40 to 59 years, with a high level of education and a high income of about $79,000. 15% of core wine drinkers have wine daily, 48% drink a few times a week, and 37% weekly. Marginal wine drinkers prefer white wine, about 46%, followed by red, 35%, and blush/rose, 19%. They are somewhat younger than core drinkers, 49% are between 40 and 59 years and have a relative high income of around $63,000. 52% drink wine two to three times a month, 30% drink once a month and 18% drink once every two to three months. Fifty to fifty-nine year olds drink 16.4 bottles per year. Twenty-one to twenty-nine year olds drink 6.6 bottles per year. The United States is arguably the best place to grow grapes in the world. The United States boasts world-famous growing areas that rival France and Italy in quantity produced and in quality of wine, as the technology and weather are extremely similar. Napa and Sonoma Valleys are also key tourist attractions, providing a constant source of customers. The wine market in California represents more than 90% of all the United Stateââ¬â¢s wine production. People drink wi... ... especially in the pop-premium market, to all wineries in California. à à à à à à à à à à Ultimately, the continued success of Americaââ¬â¢s wine industry will depend on its ability to meet all types of challenges, and continue to produce choice grapes and the finest wines. à à à à à In the short space of a generation, the American wine industry has maintained the tradition of boldness, innovation, creativeness, and success. American wineries that have been successful cannot rest on those successes, however. The industry and its competitive forces continue to change and members of the current industry will survive only if they are able to anticipate and deal with these changes. While there are significant threats to the American wine industry, there is also a large domestic and worldwide market of potential consumers who have yet to enjoy the fine taste of Americaââ¬â¢s wines. BIBLIOGRAPHY www.researchandmarkets.com libweb.sonoma.edu www.winebusinessmonthly.com www.cosmos-club.org/journals www.sierranevada.edu www.datamonitor.com www.impacteen.org www.findarticales.com/p/articles www.agmrc.org/fruits/info/winebackground.pdf
Friday, October 11, 2019
Counting cells using the pour plate method Essay
In the start of this assignment, I was told to choose one of seven other experiments to do. I chose the Counting cells using the pour plate method because I find it much easier than the other ones. In addition, I have had past experience therefore; it should be straightforward. I also have more knowledge of it than the other experiments. I will be testing the effects of various items on the growth of bacteria. I will investigate using the pour plate method in which I will be counting the cells of bacteria produced, of which are viable. The pour plate method can be used to establish the amount of microbes/mL or microbes/gram in a sample. It has the benefit of not have need of earlier arranged plate, and is usually used to examine bacterial contamination of foodstuffs. While using the pour plate method, a diluted specimen is pipetted in a sterile Petri plate, and next melted agar is tipped in and combined with the specimen. Using this technique permits for a bigger volume of the diluted specimen. This is normally in the choice of 0. 1 ââ¬â 1. 0ml. This technique yields colonies, which produce colonies all over the agar, not only on the surface. Caution has to be taken with this technique to guarantee that the organism to be counted is able to resist the temperatures linked with the melted agar. Dilution Factor The dilution factor is a number used for getting the whole number of infected cells from the observed data. Microorganisms are usually counted in the laboratory using methods like the viable plate count, where a dilution of a sample is plated onto an agar medium. Following the incubation, plates with 30-300 colonies per standard-sized plate are counted. This number of colonies was selected because the number counted is high enough to have statistical accuracy, so far low enough to avoid nutrient competition among the developing colonies. Each of the colonies is supposed to have arisen from only one cell, but this may not be true if chains, pairs, or groups of cells are not entirely broken apart before plating. The sample has to be controlled so that it consists of a number of cells in the right range for plating. If the cell number is high, the sample is diluted; but if too low, the sample is concentrated. Dilutions are carried out by careful, aseptic pipetting of a known volume of sample into a known volume of sterile water, buffer, or saline. This is mixed well and can be used for plating or further dilutions. If the number of cells is unknown, then a range of dilutions is usually ready and plated. HYPOTHESIS: I predict that the more the dilution is, the lesser the number of colonies. VARIABLES: I have considered the accuracy of my measurements and come to the conclusion hat the dependent variable is the aseptic technique, which in this case was E. coli. This is because I had to measure how much I had to put into each of the sterile distilled water bottles. I did not have to make many measurements but other than measuring, the E. coli and a sample of dilution into the next solution then transfer 1. 0cm3 into the petri dish. Obviously, other events took place among these measurements. The independent variable was the Pasteur pipettes which I had to keep changing every time I used one so that my solutions will not get contaminated. My variables are continuous. This means that each time I done the experiment I had to do the same thing over again, therefore they are continuous. APPARATUS: à Six universal bottles, or capped containers ââ¬â each containing 9. 0cm3 of sterile, distilled water à Twelve sterile Pasteur pipettes ââ¬â plugged with cotton wool 1cm3 plastic syringe, fitted with a silicon rubber connector, to attach to Pasteur pipettes à Six sterile Petri dishes à Suitable culture for counting, e. g. E. coli or sample of pasteurised milk à Supply of suitable agar medium, molten, kept in water bath at 45i C à Bunsen burner China graph pencil or spirit marker pen à Discard jar containing disinfectant Incubator at 30i C à Adhesive tape à Alcohol. Ruler The different items must be the same amount as each other and these measurements must be accurate due to incorrect results. To make sure my results are reliable I will make sure I count the cells of bacteria twice so I know if I have made any errors. HEALTH AND SAFETY: à Wear protective clothing (gloves) à Wear eye protection (safety glasses) à Tie hair up à Make sure you donââ¬â¢t throw the plastic syringe and sharpened pencil around due to people being stabbed by a pencil à Make sure hands are washed before and after the experiment, thoroughly with soap and water. Working area must be clean during work à Must be aware of contamination à Everything must be labeled correctly due to confusion and a mix up in solutions à Industrial Methylated Spirit is highly flammable to be careful à If the alcohol in the beaker catches on fire, cover the beaker with a damp cloth PROBLEMS WITH PLATE COUNTS: à They need long incubation for colonies to even show à When cell clump, they can guide to an error in counting the viable cells It is extremely simple to have too less or too many colonies on a plate to precisely measure viable count. Avoidance of squashing usually involves serial dilution TO AVOID CONTAMINATION OR OTHER PROBLEMS: Wash hands with soap thoroughly before and after experiment à Disinfect table before and after experiment à Ensure lid of the plate is not took off completely Do not even put the lid on the table so other bacteria does not get onto plate à Do not cough or sneeze on the plates Work near bunsen burner METHOD: Set up equipment. Label containers of sterile distilled water 10-1, 10-2, 10-3, 10-4, 10-5 and 10-6 and the Petri dishes similarly. Label the Petri dishes on their bases. Shake the sample thoroughly to ensure that it is evenly mixed. Then using aseptic technique, transfer 1. 0cm3 of the container labeled 10-1, using the sterile pipette. After use, place the pipette into the discard jar of disinfectant. Mix this first dilution carefully then. Using a fresh sterile pipette each time, transfer a 1. 0cm3 sample of each dilution separately to each appropriate, labelled Petri dish. Again, using aseptic technique, carefully pour cooled, but molten, sterile agar medium into each Petri dish. Swirl each Petri dish very carefully to ensure that the samples and the agar are evenly mixed. Gently move each dish in a figure of eight pattern, but do not allow the agar to spill over the edge of the dishes. Allow the agar to set, and then fasten each lid with 2 pieces of adhesive tape. Invert the dishes, and incubate at 30i C. After incubation, count the number of colonies present in a dish containing a suitable dilution. Calculate the number of viable cells present in 1. 0cm3 of the original culture. As an alternative to pipetting a 1. 0cm3 sample into each Petri dish and then adding molten medium, a 0. 1cm3 sample may be transferred to a ready poured agar plate. The sample is then spread uniformly over the surface of the agar medium using an alcohol flammed glass spreader. 1. 2. 3. 4. Following a couple of days, various sorts of microbes grow as divided colonies. Cells from separate colonies could be picked up for a subculture. IMPLEMENTING This was a very quick process in which everything had to be complete straight after another. Therefore, measurements also should have been done quickly during the experiment straight away to put into whichever solution it may have been. My results have been recorded according to how much attempts I made. In each attempt, I have shown the dilution factor and how many cells I saw in each square using the see through round scale. The see through round scale had 64 squares in it. Some squares were completely filled therefore I have written that down too. I done three replicate to ensure my results were accurate. 10-6 1422 320 1088 943. 3 My results show that as the dilution factor increases the amount of colonies decrease, as stated in my hypothesis. The decrease is shown as exponential, also there no peaks. According to my results, the values are quite variable, but as predicted. The maximum value in average is at the 10-1 dilution factor, 10688 and the minimum value in average is at the 10-6 dilution factor, 943. 3. Here are my results shown on a line graph: ANALYSING CONCLUSIONS: My hypothesis stated that the more the dilution factor would be, the lesser the number of colonies. Well, according to my results, I was correct. As my dilutions increase, my colonies decrease. This is because, during the experiment when I had to take out 1cm3 of solution from 10-2 and put it into the next, which was 10-3, the E. coli was being shared, and decreased as it was let out through the syringe. When I poured it into 10-3, I had to shake it so it was mixed properly. Subsequently, I did the same again but to the next aseptic technique, which was 10-4. Again, the E. coli was being shared. Obviously, it was lesser than it was in 10-2 because it was also being shared in 10-3 and 10-2. This is why as the dilution factor raises, the colonies fall. ANOMALOUS RESULTS: As shown by my results, I only had one error. This was in my third attempt of the experiment at dilution 10-5. It may have been due to contamination while carrying out that particular part of the experiment. For example, I may have left the lid of the plate on the table, which could have not been disinfected, therefore it picked up other bacteria. Alternatively, it could have just been due to my infective flu, I probably sneezed unintentionally on the plate, which caused the whole plate to be filled with colonies. Other reasons include my hands being dirty. Next time I will make sure I wear gloves, or I sneeze to the side if I do and I ensure that I keep the desk disinfected encase I by chance leave the plateââ¬â¢s lid n the table. However since there was only one error, I do not think it made a huge difference to the experiment since my prediction was still correct. But next time I will be aware of these little mistakes. EVALUATION I think my results were reliable since I just made one error and did not have any other anomalies. However, i think if I was to do the experiment, again, I would improve on avoiding contamination and I would do more replicates to show my results as more reliable. My results do not have a specific trend or pattern in which they decrease in, but the fact that they do not keep increasing and decreasing shows its reliability. My replicate values are not very close together; therefore tell I should have done more replicates for accuracy. I think I may have made parallax errors when counting the cells. This means I may have miscounted the results or over counted them. This may have been because of my bad eyesight or due to distraction while counting. This could have been improved to accuracy if I counted each plate 3 times at least. So the correct amount of colonies in each plate would be certain and not doubted on. On the other hand, I could have used a different method to count the cells to make it easier for me, like using a counting meter. To achieve much accurate results I think, other than avoiding contamination, I could have changed around my method a little so it could have been done quicker or much accurately. For example, I could have just left the petri dishes in the incubator for a little longer or lesser period; I could have also used a different culture for counting. If I were to do the experiment again, I would repeat it more than just 3 times so my results can show more accuracy and I can identify where/when I went wrong. Furthermore, the next time I would limit the temperature to see if that would make a difference in allowing my results to be precise and I would also avoid causing any errors. BIBLIOGRAPHY à http://www. bio. fsu. edu/courses/mcb4403L/dilution. pdf à http://filebox. vt. edu/users/chagedor/biol_4684/Methods/platecounts.html http://biology. clc. uc. edu/fankhauser/Labs/Microbiology/Meat_Milk/Pour_Plate. htm à http://www. microbiologyprocedure. com/microbiological-methods/pour-plate-method. htm à Class notes à Class hand outs à http://www. mansfield. ohio-state. edu/~sabedon/biol4038. htm. Micro Organisms and Biotechnology, John adds. Erica Larkcom. Ruth Miller (Nelson) ISBN 0-17-448269-8 à http://books. google. co. uk/books? id=AtjDUn5KfG0C&pg=PA185&lpg=PA185&dq=Counting+cells+using+pour+plate+method&source=web&ots=H1ulPxFpd3&sig=S9pvM8ulJXfrta7nuKb74VX4H5w&hl=en&sa=X&oi=book_result&resnum=10&ct=result#PPA186,M1.
Thursday, October 10, 2019
How Does Orwell Convey His Thoughts and Feelings to the Reader Essay
George Orwell conveys his thoughts and feelings to the reader in many numbers of ways. One way in which he does this, is with his use of language in the third paragraph. Although the third paragraph is very short, it is clear to see what George Orwellââ¬â¢s feelings are about the Elephant. ââ¬ËIt seemed to me that it would be murder to shoot himââ¬â¢ shows the reader that Orwell did not want to shoot the Elephant as he would feel like he had murdered someone. From this evidence, we can deduce that Orwell believes that Elephants have human characteristics otherwise; he would not say that killing an Elephant was ââ¬Ëmurder. Also in the third paragraph, when George Orwell uses the word ââ¬Ëgrandmotherlyââ¬â¢ to describe the ââ¬Ëair that elephants have. ââ¬â¢ The reader is able to see that Orwell believes that the Elephant contains human characteristics. He would not being using a word like ââ¬Ëgrandmotherlyââ¬â¢ to describe an Elephant as it is a word associated with humans. Another way in which Orwell conveys his thoughts and feelings to the reader is by his mention of the ââ¬Ëimmenseââ¬â¢ crowd. Throughout the passage, Orwell is always making a point about the crowd which has gathered to watch him kill the Elephant. When Orwell is saying things such as ââ¬Ëthe immense crowdââ¬â¢ and ââ¬Ëthe will of the faces behind meââ¬â¢ it shows the reader that Orwell feels like without the crowd, he would be unable to shoot the poor defenceless giant. It is also clear to see that Orwell is put under intense pressure by the masses that are willing him on to kill the Elephant. When Orwell says ââ¬ËThe crowd grew very stillâ⬠¦happy sigh, as of people who see the theatre curtain go up at lastââ¬â¢ shows to the reader that Orwell killing the Elephant is like a show for the thousands of Burmese people watching him. This creates pressure on Orwell as he is playing the role of a lone actor. It is as if he is the star of a one man show. As he is the only way ââ¬Ëactingââ¬â¢ he is the only one under pressure from the large crowds, which have gathered with anticipation to see him perform. The performance is Orwell shooting the Elephant. The final way in which Orwell conveys his feelings and thoughts to the reader is the tone Orwellââ¬â¢s narrative adopts. The tone adopted by the narrative is friendly, revealing yet informal. This approach helps to draw the reader in to the passage. A revealing tone is created by Orwell throughout the passage as he is always revealing that he does not want to shoot the Elephant, but the presence of the crowd is forcing him to do it. Evidence for this is ââ¬Ëit would be murder to shoot himââ¬â¢ and ââ¬ËTo come all that way, rifle in hand, with two thousand people marching at my heels, and then to trail feebly awayâ⬠¦no that was impossible. ââ¬â¢ Orwell creates a friendly tone in his passage by using words such as ââ¬Ëlaughââ¬â¢ and ââ¬Ëhappy. ââ¬â¢ Although these words may be out of context, they still show the reader that Orwell is attempting to make as much of a friendly atmosphere for the reader as possible. The final tone Orwell creates is an informal one. This is clear to see throughout the passage as Orwell is always using an informal tone. The informal tone shows to the reader that Orwell believes (thinks) that the passage does not need to be formal as the topic which he is discussing is an informal topic. To conclude, George Orwell conveys his thoughts and feelings to the reader in three different ways. These three ways, are equally revealing as they all convey Orwellââ¬â¢s many different thoughts and feelings to the reader.
Wednesday, October 9, 2019
New kingdom egyptian pharaohs Essay Example | Topics and Well Written Essays - 1000 words
New kingdom egyptian pharaohs - Essay Example As in ancient Egypt, the Pharaohs in the new kingdom, were at the top of social and political order, including control over military occupations. In addition, they helped maintain divine order in the world (PBS, n.d.). While Tutankhamun reopened the temples of Amun, Ahmose ended the war, which was begun by his grandfather, Tao II. Ahmose pampered the local administrators with gifts of land and entrusted them with the responsibility of daily rule of his country. In the earlier dynasties, also the provincial leaders gained too much power because they had land and they were allowed freedom by the Pharaohs (Phouka, n.d.). With the support of the military, he maintained the balance of power (DiPaolo, 1997). In the new kingdom, although people were educated and professionals could be found, peasants and servants remained at the bottom of the hierarchy as in ancient Egypt. Women remained confined to the house and taking care of the children (PBS). Queen Hapshepsut and Thutmose III turned Eg ypt into ancient super power (Millmore). Amenhotep III, like the kings of the ancient Egypt, built temples and statues, but he encouraged realism in art. He and his son brought about changes in the cultural identity of the people during their reign. While most royal marriages have a political motive, Amenhotep IIIââ¬â¢s marriage had none. He maintained balance of power through democracy. His son Akhenaten tried to introduce the concept of godhead, which was both monotheistic and abstract. The portraits and paintings were revolutionary and allowed creative freedom. They displayed beauty and decay in real people rather than flatter the king and his family as in ancient Egypt (Millmore, 1997). The New Kingdom Pharaohs established colonial settlements only at Tombos and the temple town of Kawa. These were not completely Egyptian sites as the local elites were allowed cultural and political autonomy provided the precious luxury goods reached the Pharaohs
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